Phosphoglucomutase

[unigirl]

Could someone please tell me the activators and inhibitors of phosphoglucomutase.

Thanks

[theprof]

What a great question for a Sunday morning!!

OK. The principal activators include metal-binding agents such as histidine, diphenylthiocarbazone and 8-hydroxyquinoline, as well as glutathione, , glucose-1,6-bisphosphate and divalent cations such as Mg++.

Inhibitors include almost all chelating agents in high concentration, nucleotides, gluconate-6-phosphate and the majority of acetates.

A chilled acetate buffer at pH 5.0 is normally the preferred method of storage but activity will deteriorate over around two months. PH limits are 4.5 and 8.5 during storage

[unigirl]

Thanks prof, that's just what i needed!

What about the molecular weight?

[theprof]

Err, you're asking a bit much now as this Caol ila 12 Year old Malt whisky has gone to my head!

All the same, if I remember correctly it's about 62,000 daltons. I'd check it if I were you.